MicroRNAs (miRNAs) are small, single-stranded RNAs that negatively regulate gene expression. A large number of studies have shown that miRNAs perform important functions in many cellular processes, including development, proliferation, differentiation, and apoptosis. The expression patterns of many miRNAs are reflective of various pathological and physiological processes. Accordingly, miRNAs are being considered as promising disease biomarkers. Therefore, we are interested in the development of analytical methods for detection and analysis of miRNAs, which is necessary for the discovery of new biomarkers or the monitoring of multiple biomarkers to improve the specificity and sensitivity of diagnostic tests.